Undergraduate Research: Wendy Hoffert and Male Mate Choice in Butterflies

Wendy Hoffert at Society of Integrative and Comparative Biology meeting January 2025 presenting her research poster

Author: Wendy Hoffert | Major: Biology | Semester: Fall 2024

My research focuses on male mate choice in butterflies and its genetic correlation
with female preferences. In our lab, we study how traits such as wing spots,
color, and pattern influence mate preferences, seeking to understand the
intricate relationships between genetic inheritance, behavior, and evolution. By
uncovering genetic links between male and female preferences, we aim to
contribute to broader conversations in evolutionary biology and genetics. This
work provides insights into how sexual selection shapes biodiversity and drives
evolutionary processes, offering a better understanding of the mechanisms that
influence species’ development and survival. I first became involved in this
project during my freshman year when I actively sought research opportunities
in genetics. Intrigued by the intersection of genetics, behavior, and evolution, I
reached out to Dr. Erica Westerman, whose lab focuses on animal behavior
including mate choice in butterflies. Under her mentorship, I began to explore
the fascinating interplay between genetics and behavior, eventually narrowing
my focus to a line of butterflies that had been bred for female preferences for
male wing spot numbers. This project allowed me to investigate whether male
preferences align with these female-driven traits, shedding light on potential
genetic links between the sexes.

Conducting this research presented both exciting opportunities and complex
challenges. A central part of my work involved running behavioral assays to
measure male butterflies’ preferences for selection-line versus wild-type
females. These experiments required patience and precision. Reliable data
depended on completing at least 20 successful assays for each male type, which
meant I had to ensure consistent experimental conditions and meticulous
attention to detail. Early on, logistical hurdles such as maintaining a healthy
butterfly population and finding adequate time for assays slowed my progress.
However, with Dr. Westerman’s guidance, I adjusted my schedule and developed
a strategy to run three assays per week. This adaptation not only ensured steady
progress but also taught me the importance of flexibility and perseverance in
research.

The technical aspects of my work provided an unexpected learning curve but
also an opportunity to develop essential skills. Handling butterflies with care
required improving my fine motor skills, and painting precise wing spots for
experimental manipulation demanded a steady hand and focus. These
experiences helped me develop greater precision and a deeper appreciation for
the intricacies of experimental design. Beyond the technical skills, I also
cultivated a stronger sense of organization, patience, and adaptability—qualities
that have not only improved my research abilities but also influenced how I
approach challenges in other areas of my life.

One of the most rewarding aspects of my research journey has been the
collaborative and supportive environment in Dr. Westerman’s lab. Her weekly
feedback sessions provided me with the guidance I needed to refine my methods
and stay on track. She also encouraged critical thinking, challenging me to
consider alternative explanations and approaches to my results. Graduate
assistant Kiana Kasmaii played a significant role in my development as well,
offering valuable advice on maintaining the butterfly selection line and
troubleshooting experimental challenges. Working alongside them has fostered a
sense of community and collaboration that has been vital to my growth as a
researcher.

Thanks to the Honor’s College Travel Research Grant, I had the incredible
opportunity to attend the Society for Integrative and Comparative Biology (SICB)
meeting in Atlanta. This experience was transformative for both my personal and
professional development. Presenting my research poster to a diverse audience
of scientists helped me refine my communication skills and gain new
perspectives on my work. Engaging with researchers from various disciplines
exposed me to innovative approaches to experimental design and inspired me to
think more creatively about my research questions. The feedback I received from
attendees reinforced the significance of my work and highlighted potential
directions for future exploration. Beyond the academic benefits, networking with
scientists and students from around the world was an inspiring reminder of the
collaborative nature of science and its potential to drive meaningful discoveries.

Looking ahead, I am excited to continue building on this research as I prepare to
defend my honors thesis in Spring 2025. This experience has not only deepened
my passion for evolutionary biology but also equipped me with invaluable skills
that will serve me well as I pursue graduate studies. The organizational skills I
developed while managing multiple experiments, the patience required for
behavioral observations, and the adaptability needed to overcome logistical
challenges will all be crucial in my future academic and professional endeavors.
Additionally, conducting this research has taught me the importance of balancing
rigorous scientific inquiry with ethical considerations, particularly in animal-
based studies. These lessons have reinforced my commitment to conducting
research that is both impactful and responsible.

My research journey has been a defining part of my undergraduate experience. It
has challenged me to grow as a scientist, a communicator, and a problem-solver.
As I continue to explore the complexities of genetic and evolutionary
mechanisms in mate choice, I am grateful for the mentorship of Dr. Westerman,
the collaboration of my lab members, and the opportunities provided by the
Honor’s College. These experiences have shaped not only my academic goals but
also my vision for contributing to the field of evolutionary genetics in the years to
come.