Biomedical Engineering
Cloning Chronicles: Investigating Macrophage Polarization with CRISPR

Cloning Chronicles: Investigating Macrophage Polarization with CRISPR

My name is Ahmed Tolba, a Biomedical Engineering major in the University of Arkansas College of
Engineering. This research was conducted under the mentorship of Dr. Chris Nelson during the Spring
2024 semester. I am a premed student with aspirations to combine patient care and scientific discovery in
my future career. Our research focused on understanding macrophage polarization using the CRISPR
dCas9-KRAB system. We aimed to create a cell line expressing dCas9-KRAB and clone specific gRNAs
(guide RNAs) targeting the inflammatory genes IL-6 and IL1β. This work sets the stage for later studies
on immune-related disorders in vitro and in vivo.

Undergraduate Research: Morghan Relich and the Role of Mitophagy Inhibition in Cancer Therapies

Undergraduate Research: Morghan Relich and the Role of Mitophagy Inhibition in Cancer Therapies

Junior, Morghan Relich, is an Honors College Grant recipient from Colorado Springs Colorado, majoring in biomedical engineering with a minor in mathematics. This past semester she was able to gain more hands-on experience in Dr. Muldoon’s lab exploring the efficacy of mitophagy inhibition as a potentiating agent for conventional chemotherapy in vitro.